Isner lost to Radwanska and Janowicz 7-5, 6-3.
An error-prone Williams lost set one of her singles match before steadying her game a bit more to hold serve and take a 4-3 set two lead.
Radwanska then held serve to pull even at 4 games all.
Serving at 40-0, Williams struck an ace down the tee to hold serve to lead 5-4.
At 5 games all, Radwanska broke serve to lead 6-5 following three forehand unforced errors from Williams.
The American broke serve to enter a second set tiebreaker then outsteadied Radwanska to level the match at one set all.
Serving to open set three, Radwanska survived a three deuce game to hold serve following two down the line forehands from Williams struck long.
The American then faced breakpoint after striking several additional baseline unforced errors.
A Radwanska backhand down the line return winner sent the Pole ahead a break of serve at 2-0.
The match then remained on serve with Radwanska leading 4-1.
Serving down 1-4 in the final set, Williams powered to a 30-0 lead before netting two crosscourt backhands to drop serve and trail 1-5.
The American took a 0-30 lead in the final game of the match with Radwanska serving. However Williams then struck a crosscourt backhand wide then hit a down the line forehand long to even the score at 30 all.
Williams struck a backhand down the line long to face match point before the Pole double faulted to reach deuce.
An ace down the tee from Radwanska set up match point two.
Williams, who had ordered an espresso coffee courtside earlier in the week to get going, netted a down the line forehand
return to end the match in her 59th unforced error after two hours and five minutes of play in a display far below her best tennis.